Yale Dermatopathology Laboratory

CLIA Laboratory Citation Details

3
Total Citations
9
Total Deficiencyies
7
Unique D-Tags
CMS Certification Number 07D0099096
Address 15 York Street Lci 504-505, New Haven, CT, 06511
City New Haven
State CT
Zip Code06511
Phone(203) 785-4094

Citation History (3 surveys)

Survey - July 1, 2025

Survey Type: Standard

Survey Event ID: C84811

Deficiency Tags: D5401

Summary:

Summary Statement of Deficiencies D5401 PROCEDURE MANUAL CFR(s): 493.1251(a) (a) A written procedures manual for all tests, assays, and examinations performed by the laboratory must be available to, and followed by, laboratory personnel. Textbooks may supplement but not replace the laboratory's written procedures for testing or examining specimens. This STANDARD is not met as evidenced by: Based on surveyor observation, record review and staff interview the laboratory failed to provide an updated procedure manual for the Hematoxylin and Eosin (H&E) staining in the subspecialty of Dermatopathology. Findings include: 1. Surveyor observation on 7/1/2025 at 11:50 AM of the Sakura Prisma Stainer, "Program- IFU#1" revealed the following for Steps 2 through 22: Step Solution Name Time (min: sec) 2 Drying Station 01:30 3 Xylene 01:30 4 Xylene 01:00 5 Alcohol 100% 01:30 6 Alcohol 100% 01:00 7 Alcohol 95% 01:00 8 Wash Station 01:00 9 Hematoxylin 02: 30 10 Wash Station 01:00 11 Differentiator 01:00 12 Wash Station 01:00 13 Bluing Agent 01:00 14 Wash Station 01:00 15 Alcohol 95% 01:00 16 Eosin 00:45 17 Alcohol 95% 01:00 18 Alcohol 100% 01:00 19 Alcohol 100% 01:00 20 Xylene 01:00 21 Xylene 01:00 22 E Xylene ---- 2. Record review on 07/01/2025 of laboratory's established "H&E Staining Sakura Prisma-G2 Combo" procedure manual H&E For DIF (IFU) protocol revealed the following for Steps 2 through 18: Step Solution Time (min:sec) 2 Wash 00:45 3 Hem 02:30 4 Wash 01:00 5 acid alc 00:30 6 Wash 00:45 7 bluing 00:30 8 wash 00:45 9 95% 00:30 10 Eosin 00:40 11 95% 00:30 12 100% 00:30 13 100% 00:30 14 100% 00:30 15 XYL 00:40 16 XYL 00:40 17 XYL 00:40 18 End Xyl ---- Note: There are discrepencies with the number of steps, type of solution, and time for the staining of the slides in the staining IFU program. 3. Staff interview on 07 /01/2025 at 11:55 AM with the Assistant Director (AD) confirmed the above discrepancies. The AD further commented that he/she failed to update the procedure manual when the new "IFU#1" program was place in use on the stainer. 4. The Statement of Deficiencies (X1) Provider/Supplier/CLIA Identification Number (X3) Date Survey Completed Name of Provider or Supplier Street Address, City, State -- 1 of 2 -- laboratory performs 2508 Immunofluroscence antibody tests annually in the subspecialty of Dermatopathology. -- 2 of 2 --

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Survey - March 14, 2022

Survey Type: Standard

Survey Event ID: QXX811

Deficiency Tags: D5401 D5411 D5481 D5407 D5413 D6096

Summary:

Summary Statement of Deficiencies D5401 PROCEDURE MANUAL CFR(s): 493.1251(a) A written procedures manual for all tests, assays, and examinations performed by the laboratory must be available to, and followed by, laboratory personnel. Textbooks may supplement but not replace the laboratory's written procedures for testing or examining specimens. This STANDARD is not met as evidenced by: Based on record review and staff interview, the laboratory failed to provide a written procedure manual that is signed and dated by the Laboratory Director (LD) before patient testing is performed. Findings include: 1. Record review on 03/14/2022 of the antibody validation binder revealed seven antibody (S100, S0X10, M5TF, HPV Low, HPV High, CK 116, Ki67) validations that were not signed and dated by the approval pathologist, 2 (HPV Low, HPV High) of which were already in use for patient testing. 2. Record review on 03/14/2022 of the laboratory procedure manual revealed no written procedure for the newly validated antibodies for the Immunohistochemistry antibody staining. 3. Staff interview on 03/14/2022 at 12:20 PM with the Laboratory Manager confirmed the above findings. 4. The laboratory performs 7,360 IHC tests annually in the specialty of pathology. D5407 PROCEDURE MANUAL CFR(s): 493.1251(d) Procedures and changes in procedures must be approved, signed, and dated by the current laboratory director before use. This STANDARD is not met as evidenced by: Based on record review, surveyor observation and staff interview, the laboratory Statement of Deficiencies (X1) Provider/Supplier/CLIA Identification Number (X3) Date Survey Completed Name of Provider or Supplier Street Address, City, State -- 1 of 3 -- failed to provide an updated written procedure manual that is signed and dated by the Laboratory Director (LD) before use. Findings include: 1. Record review on 03/14 /2022 of the 'H&E Staining Sakura Prisma-G2 Combo' Version 002 procedure with a publish date of October 2020 revealed the approval date of the procedure manual by the LD to be 02/16/2021. 2. Surveyor observation on 03/14/2022 of the H&E staining Sakura Prisma-G2 combo instrument revealed the laboratory staining program, however it was not documented in the procedure manual. 3. Staff interview on 03/14 /2022 at 12:30 PM with the Laboratory Manager confirmed that the laboratory updated the procedure manual to include the staining program, but never had the LD sign and date the updated procedure manual. D5411 TEST SYSTEMS, EQUIPMENT, INSTRUMENTS, REAGENT CFR(s): 493.1252(a) Test systems must be selected by the laboratory. The testing must be performed following the manufacturer's instructions and in a manner that provides test results within the laboratory's stated performance specifications for each test system as determined under 493.1253. This STANDARD is not met as evidenced by: Based on record review and staff interview, the laboratory failed to follow manufacturer's recommended temperature storage criteria in the specialty of pathology for the period of January 2020 through December 2020. Findings include: 1. Record review on 03/14/2022 of the 'Refrigerator Temperature Maintenance Log' for the period of January 2020 through December 2020 revealed the following: a. Acceptable temperature range for refrigerator 5850 in 2020 was 3 to 10 Degrees Celsius. b. Based on the package insert of the reagents stored in that refrigerator, the recommended storage temperature is between 2 to 8 degrees Celsius. c.

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Survey - March 1, 2019

Survey Type: Standard

Survey Event ID: 2CNM11

Deficiency Tags: D5413 D5209

Summary:

Summary Statement of Deficiencies D5209 PERSONNEL COMPETENCY ASSESSMENT POLICIES CFR(s): 493.1235 As specified in the personnel requirements in subpart M, the laboratory must establish and follow written policies and procedures to assess employee and, if applicable, consultant competency. This STANDARD is not met as evidenced by: Based on record review and staff interview, the laboratory failed to have a policy in place to assess the competency of all laboratory personnel. Findings include: 1. Review of the laboratory's competency records on 3/1/19 revealed the following: a. The laboratory did not have policy in place to assess the competency of the clinical consultant, technical supervisor, and general supervisor. b. Competency documentation for the above laboratory personnel was not available. 2. Staff interview with the laboratory manager on 3/1/19 at 12:50 PM confirmed the laboratory did not have a policy in place to assess the competency of the above laboratory personnel and they were not assessed. D5413 TEST SYSTEMS, EQUIPMENT, INSTRUMENTS, REAGENT CFR(s): 493.1252(b) The laboratory must define criteria for those conditions that are essential for proper storage of reagents and specimens, accurate and reliable test system operation, and test result reporting. The criteria must be consistent with the manufacturer's instructions, if provided. These conditions must be monitored and documented and, if applicable, include the following: (1) Water quality. (2) Temperature. (3) Humidity. (4) Protection of equipment and instruments from fluctuations and interruptions in electrical current that adversely affect patient test results and test reports. Statement of Deficiencies (X1) Provider/Supplier/CLIA Identification Number (X3) Date Survey Completed Name of Provider or Supplier Street Address, City, State -- 1 of 2 -- This STANDARD is not met as evidenced by: Based on record review and staff interview, the laboratory failed to ensure temperature readings on instrumentation were recorded in the subspecialty of histopathology and oral pathology. Findings include: 1. Record review of the laboratory 2018 and 2019 maintenance logs on 3/1/19 revealed the following: Log Name Temperature range (Celsius) a. Waterbath Serial # (SN) 9507808 38-45 b. Cryostat SN 0583/10.2006 -20- -30 c. Embedding Center SN51013034-0914 58-64 d. Waterbath #6 Station 38-45 e. Waterbath SN C&AU070413 38-45 f. Embedding Center# Station 58-64 g. Oven Routine 78-82 Overnight 30-40 The above logs contain daily checkmarks with a translation equaling 'within range'. Documentation of the actual temperature record was not available. 2. Staff interview with the laboratory manager on 3/1/19 at 11:45 AM confirmed the above findings. 3. The laboratory performs 93,949 pathology tests annually. -- 2 of 2 --

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